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ATCC
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Image Search Results
Journal: bioRxiv
Article Title: Kinetic proofreading as a mechanism for transcriptional specificity in living human cells
doi: 10.64898/2026.03.17.711757
Figure Lengend Snippet: (A) Schematic for transcriptional regulation by ligand-inducible nuclear receptors. (B) Experimental designs for either 2 or 4h activation of GR, RXR, RARa, and PPARy nuclear receptors using synthetic ligands in human bronchial epithelial cells. (C) Volcano plots showing differentially expressed genes by RNA-seq after activation of nuclear receptors 2h post-treatment (dashed lines indicate adj p-val threshold of 0.05). (D-E) RNA-seq genome browser tracks (D) and read count values (E) at the ERRFI1 and MYH9 loci at the indicated ligand treatments. (F) GR-ChIP and H3K27ac-ChIP genome browser tracks at ERRFI1 in the absence and presence of Dex. (G) Summary of GR transcription factor acting as a specific transcription factor for ERRFI1 and non-specific transcription factor for MYH9 gene.
Article Snippet: Cells were cultured in Airway Epithelial Cell Basal Medium (ATCC, PCS-300-030) supplemented with 1% penicillin/streptomycin and
Techniques: Activation Assay, RNA Sequencing
Journal: bioRxiv
Article Title: Acid stress modulates metabolo-inflammatory pathways in oral epithelial cells
doi: 10.64898/2026.03.16.711383
Figure Lengend Snippet: Schematic representation of the experimental design to investigate the impact of acid stress on oral epithelial cells (OECs) and Toll-like receptor (TLR) agonist stimulation. pH Conditioning : OECs (80% confluence) were cultured in acidified (pH:=:3.0) or complete growth media (pH:=:8.0) for 24h. Morphometric Analysis ( a ): Brightfield micrographs and a machine-learning based image analysis pipeline were used to assess changes in cellular morphology. TLR Agonist Challenge : Cell cultures were subjected to either 100 ng/mL flagellin (TLR5 agonist) or 1 mg/mL Pam3CSK4 (TLR2/1 agonist) for 2-, 6-, or 24h. Molecular profiling ( b ): was conducted on OEC RNA collected after 6h TLR agonist challenge using NanoString® nCounter® technology followed by pathway analysis using the Gene Ontology knowledgebase. A TGF- β ELISA ( c ) was performed on OEC supernatants collected 2-, 6-, and 24h post TLR agonist stimulation. Figure made with Biorender.com
Article Snippet: Low-passage mixed-donor,
Techniques: Cell Culture, Enzyme-linked Immunosorbent Assay